Objective: NMR is a method of choice to perform structural determination of these news neuropeptides. But in fact, this apparent simple approach is not so trivial for two reasons. i) Neuropeptides are usually small peptides lacking a defined secondary structure in aqueous environment. Consequently the structures have to be determined using a membrane model. ii) Furthermore, hydrophobic solvents, charged or zwitterionic micelles, are commonly used, but sometimes lead to different structures.
We first plan to study some standard neuropeptides, synthesized by Partner 2. We define some structural parameters in the presence of phospholipids as well as the best media for the peptide folding. Three-dimensional structures of the neuropeptides, from eel, oyster, cuttlefish and coral, are then determined in several media.